CD59 antibody

Catalog No. ABIN119472

This anti-CD59 antibody is a Rat Monoclonal antibody detecting CD59 in WB, FACS, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for CD59 antibody (ABIN119472)

Target: CD59

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YTH53-1

Product Details anti-CD59 Antibody

Specificity: This antibody recognises the CD59 cell surface antigen. Clone YTH53.1 has been shown to block the normal function of CD59.

Purification: Affinity Chromatography on Protein G

Immunogen: Human Peripheral Blood T cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma line.

Isotype: IgG2b

Application Details

Application Notes: Western Blot. Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/5 - 1/10, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Placenta. Removal of sodium azide is recommended prior to use in functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: CD59 is an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin

Gene ID: 966

NCBI Accession: NP_000602

UniProt: P13987

Pathways: Complement System